■ 基本信息
復制子: | p15A |
質粒大小: | 3941bp |
原核抗性: | Amp,Kan |
克隆菌株: | DH5a |
培養(yǎng)條件: | 37度 |
5'測序引物: | 根據序列設計 |
3'測序引物: | 根據序列設計 |
備注: | 豐富的酶切位點 |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 基因模板 |
片段類型: | cDNA
|
片段物種: | 空載體
|
原核抗性: | Amp,Kan |
真核抗性: |
|
熒光標記: |
|
■ 質粒簡介
pACYC177 is an E. coli plasmid cloning vector containing the p15A origin of replication (1-4). This allows pACYC177 to coexist in cells with plasmids of the ColE1 compatibility group (e.g., pBR322, pUC19). It is a low copy number vector, at about 15 copies per cell (5), but can be amplified with chloramphenicol or spectinomycin.
Enzymes with unique restriction sites are shown in bold type and enzymes with two restriction sites are shown in regular type. Location of sites of all NEB restriction enzymes can be found on the NEB web site (choose Technical Reference > DNA Sequences and Maps). Restriction site coordinates refer to the position of the 5′-most base on the top strand in each recognition sequence.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列可參考（具體以測序為準）下載：
ZK119pACYC177大腸克隆質粒.txt
