■ 基本信息
啟動子: | T7 |
復制子: | ori |
終止子: | T1,T2 |
質粒分類: | 植物系列，gateway載體 |
質粒大小: | 4291bp |
質粒標簽: | ccdB |
原核抗性: | Zeo |
克隆菌株: | DB3.1 |
培養(yǎng)條件: | 37度 |
表達宿主: | 植物細胞 |
5'測序引物: | M13F:TGTAAAACGACGGCCAGT |
3'測序引物: | M13R:CAGGAAACAGCTATGACC |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 基因模板 |
片段類型: | ORF,GateWay |
片段物種: | 空載體 |
原核抗性: | Zeo |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
pDONRvectors are Gateway?-adapted vectors designed to generate attL-flanked entry clones containing your gene of interest following recombination with an attB expression clone or an attB PCR product. Once you have created an entry clone, your gene of interest may then be easily shuttled into a large selection of expression vectors using the Gateway? LR recombination reaction.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK874pDONR221Zeo大腸克隆質粒.txt
